The secondary oocytes from Saanen dairy goats and 4-32 cell embryos from Shaanbei black goats were used as cytoplast donors and karyoplast donors respectively.
选用陕北黑山羊的4 - 32细胞胚和萨能奶山羊的次级卯母细胞分别作核供体和胞质供体。
We first observed that the head ofmature tentacle contained transfer cells which were pellet shape, thickness cytoplast, large nucleolus, developing endoplasm, small vacuole and thick cell wall.
The paper mainly reviewed several factors on influencing somatic cloning of pigs, which involve in cytoplast recipients, donor cells, micromanipulation, activation and reconstituted embryo transfer.